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KMID : 0352419940130020182
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Keimyung Medical Journal
1994 Volume.13 No. 2 p.182 ~ p.191
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Detection of Mycobacterium tuberculosis DNA in pleural biopsy tissues and pleural fluids by Polymerase Chain Reaction(PCR)*
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한승범
허정숙/전영준/백성덕/백원기/서민호
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Abstract
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Polymerase Chain Reaction(PCR) amplification was used to detect Mycobacterium tuberculosis DNA in pleural biopsy tissues and pleural fluids from patients suspected as tuberculosis. Oligonu cleotide pairs for M. tuberculosis IS6110 DNA were used
as
primers and M. tuberculosis H37Rv strain was used as a positive control. Amplified products of 123 base pair fragments were detected by agarose gel electrophoresis and by Southern blotting with digoxigenin-labeled M. tuberculosis IS6110 DNA
probe.
DNA
were isolated directly from the clinical specimens by bead-beating technic.
Positive results were obtained in 13 of 15(86.6%) pleural biopsy tissues and 17 of 21(80.9%) pleural fluids. All the positive results were positive both in agarose gels and in Southern blottings. The relationships between the biopsy findings and
PCR
results were evaluted. Two「tuberculosis」, 10「granulomatous inflammations」, and one「non-made」findings of biopsy results were all positive by PCR. But one「chronic inflammation」and one「inadequate specimen」findings of biopsy results were
all
negative by PCR.
In conclusion, these results showed that the PCR provides a very sensitive and efficient tool for the accurate and rapid diagnosis of tuberculosis infection.
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KEYWORD
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